vegf elisa kits  (Elabscience Biotechnology)

Journal: International Journal of Biological Sciences

Article Title: Targeting NCAPG with Arundanine Suppresses Neutrophil/NET-Mediated Immune Evasion and Enhances CD8 + T Cell Function in Lung Squamous Cell Carcinoma

doi: 10.7150/ijbs.134668

Figure Lengend Snippet: The E2F8/NCAPG axis affects TAN phenotype and tumorigenesis in mice. KLN205 cells stably transfected with E2F8 KD and NCAPG OE were implanted into DBA/2 mice subcutaneously. (A) Growth rate of subcutaneous tumors in mice over 28 days. (B) Representative images and weight of in mice on day 28. (C-D) Populations of iNOS + Ly6G + (N1) and Arg1 + Ly6G + (N2) neutrophils in tumor tissues determined using double label immunofluorescence staining. (E) Concentrations of IL-8 and VEGFA in tumors determined using ELISA kits. (F) Levels of p-SMAD2 and p-SMAD3 in tumors determined using WB analysis. (G) Cell apoptosis in tumors determined using TUNEL assay. (H) Expression of E2F8 and NCAPG in tumor tissues analyzed using IHC. (I-K) Flow cytometry detection of the proportion of total CD3⁺CD45⁺CD8⁺ T cells (I), GZMB⁺ (J) or TNF-α⁺ CD8⁺ (K) T cells in tumor tissues. (L) KLN205 mouse lung cancer cells were subjected to E2F8 KD and NCAPG OE transfections and then implanted into DBA/2 mice via tail vein injection, followed by Kaplan-Meier analysis for 70-day survival rate of mice in each group. Data are expressed as the mean ± SD and shown as dot plots with whiskers representing SD. In vitro assays represent n = 6 independent biological replicates. For animal experiments, n = 6 biologically independent mice were analyzed per group, except n = 10 per group in panel L. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The contents of tumor necrosis factor α (TNF-α), interleukin-8 (IL-8), and vascular endothelial growth factor A (VEGFA) in TAN supernatant and tumor tissue homogenates were quantified using ELISA kits for Human IL-8 (E-EL-H6008, Elabscience Biotechnology Co., Ltd.), Human TNF-α (E-EL-H0109, Elabscience), Human VEGFA (E-EL-H0111, Elabscience), Mouse KC (IL-8, MBS8579382, Mybiosource, San Diego, CA, USA), and Mouse VEGFA (E-EL-M1292, Mybiosource).

Techniques: Stable Transfection, Transfection, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Analysis, TUNEL Assay, Expressing, Flow Cytometry, Injection, In Vitro

Journal: Bioactive Materials

Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration

doi: 10.1016/j.bioactmat.2026.02.059

Figure Lengend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

Article Snippet: ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and HGF (Cat. No. E-EL-R0496) were purchased from Elabscience (Wuhan, China).

Techniques: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing